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Microbiology and Flow Cytometry

This material was originally published in the

Purdue Cytometry CD-ROM Series

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Introduction	The Purdue University Cytometry Laboratories (PUCL) recognizes that microbiology is one of the fastest growing areas in the cytometry field. The purpose of this section of the Purdue CD-ROM series is to maintain a forum to share information regarding flow cytometric applications in microbiology. We welcome contributions to future volumes.
Bacterial Viability	David Mason and Vanya Gant F. Paul, R. Jepras, D. Hynes, A. Smith and B. Marken Dave Duncan, Peggy Ooi and Robert Zagursky Roger Pickup and Jonathan Porter Jim Cornelius and George Babcock Beth Harman and Bill Rutherford Kuo-Ping Chiu, Diana Davis and Craig Hixson Application of flow cytometry to the study of antibiotic activity. Activity of common oral antiseptics against bacteria assessed using the oxonol DIBAC4(3). Flow cytometric analysis of gram-negative bacterial cells. Combination of flow cytometry and fluorescent probes to determine bacterial viability. Antibiotic sensitivity using LIVE/DEAD BacLight Viability kit. Viability of dried fermentation samples. Antimicrobial susceptibility testing using flow cytometry.

Molecular Techniques	Jean-Christophe Thomas, Marcel Desrosiers, Richard Villemur and Yves St-Pierre E. Tinsley, R.Jepras, F. Paul and E.Carter Roger Pickup and Jonathan Porter Quantitative flow cytometric detection of specific microorganisms in soil samples. A flow cytometric study of stationary phase gene expression in E.coli using lacZ reporter gene fusion. Flow cytometric detection of specific bacterial genes using in situ PCR.

Bacterial characterization	Hazel Davey Jim Ho Gerhard Nebe-von Caron, P. Stevens and R. Andrew Badley Roger Pickup and Jonathan Porter Flow cytometric analysis of microorganisms stained with fluorescent dyes. Real time detection of biological aerosols. Bacterial detection and differentiation by flow cytometry and fluorescent probes. Immunofluorescent flow cytometry and cell sorting of bacterial populations.

Food Microbiology	Richard Raybourne, Mary Lou Tortorello and Gabrielle Roth Sergio Sgorbati and Silvia Barbesti Rapid detection and enumeration of food-borne pathogens. Rapid flow cytometric characterization of Escherichia coli.

Aquatic Organisms	Betsy Robertson and Don Button Dominique Marie, Frederic Partensky and Daniel Vaulot Dominique Marie, Daniel Vaulot, Frederic Partensky and Stephan Jacquet Jaume Comas-Riu and Florencio Carretero Hazel Davey Michael Sieracki and Terry Cucci Aquatic bacteria. Flow cytometry in oceanography. Analysis of marine picoplankton by flow cytometry. Autofluorescence of cyanobacteria. Algae autofluorescence. Plankton analysis by flow cytometry.

Information and images from the 1997 Macroalgal Workshop - Portugal

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