Help: FACScalibur problems
Christian Schütt (100731.1123@compuserve.com)
Thu, 30 Jan 1997 10:54:26 -0500
Dear flowers!
We want to analyze marine bacterial populations using flowcytometry.
Because we are new in this field, there are a lot of problems. One problem
occurs, when putting the PMs to about 500 (which is 50% of maximum - and
necessary! for stained bacteria) we get (more or less) huge clouds of
signals even with (particle-free) 0.2 µ filtered A. dest. Shall we filter
the sheath-fluid again (we use BD sheath fluid). Is it electronic noise?
Does anybody have an idea?
Gunnar Gerdts
Biologische Anstalt Helgoland
Dept. Marine Microbiology
27483 Helgoland
Germany
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
