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Problem Statement: In wanting to
determine the minimally infective dose
(oral route in a neonatal mouse model as
well as in a human colon tumor cell line) of
cryptosporidium oocysts, we must obtain
highly accurately sorted and calibrated
pure cyst suspensions from crude density
gradient preps. Main contaminants in
crude are yeasts.
We have a highly specififc FITC-MAB,
fluor tagging of cysts has been well
worked out.
We have a Coulter Epics Elite equipped
with the SPE fast sorter and autoclone,
we are experienced sorters. ( We also
have a Meridian ULTIMA confocal scope
as an adjunct tool)
My inquiry is about:
1. Does anyone have experience with flow
sorting and/or confocal mapping of
protozoan oocysts, about potential
problems and technical tricks, etc?
2. Would FITC-MAB tagging and flow
sorting of oocysts have an adverse
impact on cyst viability, infectivity
and virulence?
3. Do I need to worry about aerosol-borne
infectious control.
Any advice or dialogue will be greatly
appreciated.
J. Peter Bercz
USEPA/NERL/EERD Cincinnati
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web