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I'm looking for a good method for staining bone marrow cells for
simultaneous cell cycle analysis and 3-color surface
immunofluorescence. Antibodies are FITC-, PE-, and biotin-conjugated.
I'm using a FACStarPlus with Argon, He-Ne, and UV lasers. Can anyone
suggest a good DNA stain and avidin conjugate, and staining method
that will work for this configuration ?
Steve Neben
Genetics Institute
Cambridge, MA
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web