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Partec’s Flow Museum
FCM HISTORY
Historic information on the first fluorescence based FCM instruments including manuals and patents.
Partec Flow Museum: ICP 11 (1968/69)
Worldwide First Fluorescence based FCM System
First commercial Flow Cytometer
Developed by Göhde/Partec in 1968 Distributed by Phywe AG, Göttingen
Technical Data
Flow Chamber
- Sample Speed: 2ml/min
- Max. Cell Concentration: 30,000/ml
- Light Source: 100W HBO UV Lamp
- Köhler Illumination
- Objective: Zeiss Neofluar 100/1.30
- Photomultiplier: green-red sensitive
Electronics
- PDP 11 Computer
- High Voltage: 250 … 1500 V
- Amplification: ×1; ×2; ×5; ×10 with threshold
- Multichannel Analyser:
- 128 Channels
- Max. 99,999 Pulses per Channel
- Line Printer
- Controls:
- Average cell count / time
- Absolute cell count
- Constant Light
- Oscilloscope for Incoming Signals
- Oscilloscope for Memory Content
- Dead Time Fraction
- Lamp Voltage
- Lamp Power
- Power Supply: 220V, 50 Hz, 5 A
- Desktop Unit:
- 700 × 250 × 600 mm (L×W×H)
- Weight: 20 kg
- Electronics Unit:
- 600 × 600 × 1500 mm (L×W×H)
- Weight: 60kg
Related Documents
- ICP 11 Technical Brochure (1969)
- ICP 11 Product Brochure (1969)
- Impulscytophotometrie (1973) (Pulse Cytophotometry)
- List of Scientists and Research Institutes having used the ICP 11 from 1968-1973
Sorted by application - Early FCM Publications
- List of Conferences with Scientific Presentations on the ICP 11
- Patent DE 1815352
- Patent GB 1300585
- Patent US 3,738,759
Partec Flow Museum: ICP 22 (1975)
2nd Generation of ICP System:
Flow Cytometer with up to 3 Optical Parameters
Technical Data
Flow Chamber
- sheath flow principle
- 200 nm orifice diameter
- suspension rate max. 8ml/min
- rinsing liquid consumption max. 40ml/min
- max. cell concentration: 30,000/ml
- max. sample rate 60,000 cells/min
Optics
- Light Source: high pressure mercury lamp 100W (HBO 100W 2, OSRAM) with separate highly stabilized lamp current supply
- Köhler Illumination
- Excitation filter: selectable according to application, for example BG12 + (BG38 + KG1)
- Reflector and barrier filter: selectable according to application, for example for 2 parameters FT580 + interference filter 510
- Objective: Zeiss Neofluar 63/1, 25 oil
- Photomultiplier: exchangable; standard model green-red sensitive (S 20 RCA, C 7164 R)
- High Voltage 0-1000 V, continuously adjustable
Fluidic System
- Low noise vacuum piston pump, driven by a Ferraris motor
- Tubing: 3 electrically checked valves for rinsing liquid, drainage, and drainage system ventilation
Indicator Electronics
- oscilloscope for signal observation, 2 parameters per screen
- 2 LED arrays to indicate average illumination and lamp alignment
Data Processing Electronics
- depending on configuration
Dimensions
- Detector Instrument (Desktop Unit): 400 × 430 × 480 mm (L×W×H) Weight: 25 kg
- Electronic Cabinet: 600 × 600 × 1500 mm (L×W×H) Weight: 50kg
- stabilized lamp current supply: 215 × 330 × 230 mm (L×W×H) Weight: 11.5 kg
Electrical Connection
- alternating current 220 V 50 Hz
- current required detector system 0.35 A electronic cabinet 0.70 A
- Adaption transformer 110/220 V AC, 50-60 Hz
Related Documents
- ICP 22 Brochure (1975)
- Impulscytophotometrie (1973)
- Patent DE 1815352
- Patent GB 1300585
-
- Patent US 3,738,759
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