I am attempting to measure apoptosis and necrosis within a population
of vascular smooth muscle cells which have been isolated from a rat
aorta.The cells were isolated by a 12hr collagenase/elastase
digestion an then used immediately for a two colour annexin V vs PI
experiment. The results are quite variable within the triplicates of each
experiment , which is quite worrying. The conditions such as cell number
incubation times etc. are the same for each experiment.
Any suggestions to improve the variability??
Thanks
James S. Clark PhD.