Re: HLA TYPING B27 BY FLOW

Vivian Wu (ffwu@unixg.ubc.ca)
Sat, 19 Apr 1997 17:55:23 -0700 (PDT)

We routinely screen HLA B27 by BD reagent. A small percentage (approx.
3-5%) requires further confirmation by serological typing or an additional
backup flow typing by a second Mab. 90% of these turn out to be B7 CREG.
We have tried One Lambda, Serotec and recently Immunotech B7/B27
reagents. We have been very impressed with Immunotech B7/B27 reagent. One
is able to distinguish B7 CREG vs B7+B27+ results quite easily.

Vivian Wu (e-mail: ffwu@unixg.ubc.ca)
Immunology Lab
Vancouver Hospital & HSC
B.C. Canada
==================================================================
On Fri, 18 Apr 1997, Andrew Lister wrote:

>
> ernest stapleton wrote:
> >
>
> > If any lab does screen for B27 there are other antibodies available
> > (they also cross react with B7), namely Serotec and Becton Dickinson.
> >St. John's NFLD
>
>
> In addition to Serotec and BD, you may be interested to check
> Coulter/Immunotech's IOTest HLA-B27/HLA-B7. This product is not
> available in the USA, but I don't think that the restriction applies to
> Canada.
>
> > Our laboratory has also evaluated B27 typing by flow and have found
> > there is no monospecific antibody to B27 yet. The one lambda clone
> > also reacts with most hla B44 positives as well. Other labs have also
> > found this cross reaction.
>
> The combo reagent should help with the B7 cross reactivity
> problem.
>
> See //WWW.COULTER.COM/ss000212.htm#HLA-B27-HLA-B7/ if you are
> interested.
>
> Regards, Andrew Lister.
>