Flow Cytometry and Microbiology

Flow Cytometry and Microbiology

Introduction	The Purdue University Cytometry Laboratories (PUCL) recognizes that microbiology is one of the fastest growing areas in the cytometry field. The purpose of this section of the Purdue CD-ROM series is to maintain a forum to share information regarding flow cytometric applications in microbiology. We welcome contributions to future volumes.





From Volume 2
Bacterial Viability NEW!	Application of flow cytometry to the study of antibiotic activity. David Mason and Vanya Gant Activity of common oral antiseptics against bacteria assessed using the oxonol DIBAC4(3). F. Paul, R. Jepras, D. Hynes, A. Smith and B. Marken Flow cytometric analysis of gram-negative bacterial cells. Dave Duncan, Peggy Ooi and Robert Zagursky Combination of flow cytometry and fluorescent probes to determine bacterial viability. Roger Pickup and Jonathan Porter Antibiotic sensitivity using LIVE/DEAD BacLight Viability kit. Jim Cornelius and George Babcock Viability of dried fermentation samples. Beth Harman and Bill Rutherford Antimicrobial susceptibility testing using flow cytometry. Kuo-Ping Chiu, Diana Davis and Craig Hixson

Molecular Techniques	Quantitative flow cytometric detection of specific microorganisms in soil samples. Jean-Christophe Thomas, Marcel Desrosiers, Richard Villemur and Yves St-Pierre A flow cytometric study of stationary phase gene expression in E.coli using lacZ reporter gene fusion. E. Tinsley, R.Jepras, F. Paul and E.Carter Flow cytometric detection of specific bacterial genes using in situ PCR. Roger Pickup and Jonathan Porter

Bacterial characterization	Flow cytometric analysis of microorganisms stained with fluorescent dyes. Hazel Davey Real time detection of biological aerosols. Jim Ho Bacterial detection and differentiation by flow cytometry and fluorescent probes. Gerhard Nebe-von Caron, P. Stevens and R. Andrew Badley Immunofluorescent flow cytometry and cell sorting of bacterial populations. Roger Pickup and Jonathan Porter

Food Microbiology	Rapid detection and enumeration of food-borne pathogens. Richard Raybourne, Mary Lou Tortorello and Gabrielle Roth Rapid flow cytometric characterization of Escherichia coli. Sergio Sgorbati and Silvia Barbesti

Aquatic Organisms NEW!	Aquatic bacteria. Betsy Robertson and Don Button Flow cytometry in oceanography. Dominique Marie, Frederic Partensky and Daniel Vaulot Analysis of marine picoplankton by flow cytometry. Dominique Marie, Daniel Vaulot, Frederic Partensky and Stephan Jacquet Autofluorescence of cyanobacteria. Jaume Comas-Riu and Florencio Carretero Algae autofluorescence. Hazel Davey Plankton analysis by flow cytometry. Michael Sieracki and Terry Cucci

Information and images from the 1997 Macroalgal Workshop - Portugal

Note: These data have been provided by colleagues worldwide. Appropriate acknowledgement should be made when material is used.
The images at the top and bottom of each page were generously provided by Dr. Allan E. Konopka, Department of Biological Sciences, Purdue University. Top image: Spirochetes; Bottom image: Bacillus anthracis.

If you have any comments or suggestions regarding this section, please email Stephanie Sincock, sincock@flowcyt.cyto.purdue.edu
If you have comments or suggestions regarding the intellectual content of the sections, please send email to the primary author(s).

CD-ROM Vol 4 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907, Phone: (765) 494-0757 FAX: (765) 494-0517, Web http://www.cyto.purdue.edu, EMAIL cdrominfo@flowcyt.cyto.purdue.edu