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I've done a couple CD34 selections on G-CSF mobilized normal-donors with
the Baxter ISOLEX system and have never experienced such results. I've
observed that the non-target cells that are captured are primarily
monocytes (CD14+). I almost never see any CD19+ positive events.
It's common to see CD34+CD19+ events in the bone marrow but I've never
observed these cells mobilized to the peripheral blood. Aftter G-CSF, most
are CD34+CD33+.
What was the CD34% of the product? Were these cells also CD45dim? Were
all the events in the selected fraction "lymph-sized" based on scatter?
What was the circulating peripheral CD34/ul count?
Drop me a note at tfarley@computer.net
Maybe I can be of some help
Dr. Timothy Farley
New York Blood Center Clinical Services
Valhalla, NY
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web