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We often do T-cell lymphocyte subset analysis after Cd34 collection.
The reason being that most of our allogeneic transplants are T-cell
depleted. We however use Campath 1 which often depletes other cell
lines as well. In our experience there is a huge drop in the T-cell
population but we have not seen the huge increase in CD19+ cells that
you have seen. We also haven't experienced the CD3+/CD19+ population
you describe.
I hope this has been helpful. I would be interested to hear what
others have experienced.
Good luck,
Glenda Davison
Dept.Haematology Groote Schuur Hospital
University of Cape Town
South Africa
email: gmd@samiot.uct.ac.za
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web