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>FACSort/PowerMac
>To FACSort/FACScan users,
>
>We recently upgraded our FACSort computer from an HP system to a PowerMac
>7600.
> In general we have been happy with this, although CellQuest could still has
>some way to go to take advantage of all Macs have to offer (but is better than
>Lysis II in most ways). However, we are having a sporadic, but persistent and
>annoying problem - when attempting to connect to the cytometer from
>withing the
> program we often get a message saying that the cytometer cannot be found.
>This is despite turning the FACS on before the computer, etc. This can go on
>for hours before it decides that the FACS really is there and lets us
>access it
> for aquisition. BD suggested reloading the software, which had no
>effect, nor
> did rebuilding the desktop. Our computer consultant has suggested that
>fragmentation of the hard disk is to blame, and that the recent high humidity
>in Sydney may also be implicated.
>
>Has anyone else had this problem with their Mac/FACS interface? We use Macs
>exclusively in our institute (with the exception of the old HP) and the
>7600 is
> networked but the harddisk cannot be accessed by other computers without
>first
> checking that the FACSort is not in use. Any suggestions would be very
>welcome.
>
>Thanks in advance,
>
>Michelle Miller
>Johnson and Johnson Research Labs
>Sydney, Australia
>email: jjphar13@angis.su.oz.au
Like for any other problem with Mac software/hardware I would try the
following in the order mentioned:
1. Rebuild the desktop (use a utility like TechTool 1.1.2 (freeware) by
MicroMat, instead of the MacOS build-in option, as this will delete the old
desktop file, instead of rebuilding the existing one)
2. Zap the parameter RAM (again use TechTool).
3. Restart with extensions off (except the inits/cdevs neccesary for
CELLQuest to run)
4. Reinstall BD software (remember to trash BD inits/cdevs and preferences) .
5. Run a harddisk check program (better Norton Utilities, than Apples Disk
First Aid) from a floppy disk.
6. Low level reformat the boot disk (internal harddisk), update the
harddisk driver and reinstall system software and BD software.
Good Luck,
Ulrik Sprogoe-Jakobsen
Dept. Clinical Immunology
Odense University Hospital
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web