 |
 |
 |
 |
 |
Yes it will certainly work, I've been doing it for years.
I use the waste tank for ETOH, and still use a transparent container
for waste.
(easy to see if it is about to overflow into the house vacuum line...)
Both tanks are online and pressurised, and I use a 3-way lauer-fit
valve to select 70% ETOH or saline.
NOTE:
Be very careful not to run the ETOH into the sheath tank, as the saline
will precipitate!
I also add an extra 1" 0.22u syringe filter into the line immediately
after running ETOH, which has several effects:
- Extra filtering of the saline, just in case.
- The small filter-full of air stops the saline & ETOH from mixing
in the line, so salt precipitation is not a problem.
- One minus, it changes the pressure balance so that the sample
differential must be reduced by about half with extra filter in.
I got this idea from Frank Battye of WEHI, with much appreciation!
Frank - you may have some comments; are you still using clear filter
holders as fluid reservoirs?
Cheers, Joseph.
-- Joseph Webster Flow Cytometry Facility Centenary Institute
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web