PI fluorescence ratios
ssmurray@student.umass.edu
Mon, 07 Jul 1997 16:14:21 -0400
We are using Propidium Iodide to stain the DNA of fish and chicken blood
cells and then using the fish/chicken emission ratios to calculate the
amount of DNA in the fish cells. All this is being done on a
Becton-Dickinson FACScan with Cellfit software. We have noticed that the
FL2H (585 +/- 21 nm bandpass) and the FL3H (650 nm longpass) fish/chicken
ratios give us different answers. FL3H consistently gives us a lower ratio by
5-10%. Shouldn't they give us the same ratio?
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
