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In my limited analysis on a Coulter XL and an Elite, distilled water gives a
different FSvsSS pattern with less separation of monos from lymphs
(everything falls down and to the left) in Q-prepped blood samples. I
hypothesized at the time that some sort of hypotonic/hypertonic gig was
going on at the sheath/sample interface...so I stuck with the sheath fluid.
Ray Biagini
Research Toxiciologist
CDC/NIOSH
DBBS, ABB, IRS
Robert A. Taft Laboratories
4676 Columbia Parkway
Cincinnati, OH 45226
REB4@NIOBBS1.EM.CDC.GOV
Voice (513)533-8196
Paperless Fax (513)533-8400
Fax (513) 533-8510
/*- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Eric Martz, Professor of Immunology emartz@microbio.umass.edu
Dept Microbiology Voice: 413-545-2325 FAX: 413-545-1578
Morrill IVN 203, Box 35720, Univ Massachusetts, Amherst MA 01003-5720
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -*/
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web