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At 1024 channel resolution, the resulting files are more than twice
as big as for 8-bit data. Not only is there an extra byte per param-
eter but, with 4 times as many channels, 4 times as many events need
to be recorded to give decent histograms with good statistics (de-
pending on the dispersion of the data set). Unless, of course, you
rebin the data down, but then why not just collect 8-bit data in the
first place? Disk space is cheap these days (less than $1/mB for
many good disks) but who wants to wait while your computer grinds
through a 1024 X 1024 channel contour plot?? Log amp transfer
functions aside, momentarily, most of the software I've seen usually
rebins to 64 X 64 channel bivariates or maybe 128 X 128 at most!
On the point of the transfer function - since Parks, et al, used
beads in a flow cytometer, as opposed to pulses from a pulse
generator, isn't the majority of the fluctuation readily attributable
to the cv of the measurement of the fluorescence intensity?
Robb Habbersett
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web