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Author: Lupton-J-R. Coder-D-M. Jacobs-L-R.
Title: Influence of luminal pH on rat large bowel epithelial cell
cycle.
Source: Am-J-Physiol. 1985 Sep. 249(3 Pt 1). P G382-8.
Several tricks included: diothiothreitol to cut up the mucus, use of an EDTA soak to
loosen cell-cell junctions followed by vibration to dislodge epithelium, washing to
get rid of debris. Ethanol seemed to work for a fixative; PI worked quite well for
staining: good G0,1 CVs were 4%.
It's a shitty business, but somebody has to do it.
Dave Coder
Internet: dcoder@u.washington.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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