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Question? Is it possible that the antibody on the flask is
remaining on the cells and blocking any subsequent staining?
We would like to develop the cells with a second step
reagent, but to what? Is the anti-CD 34 a mouse? rat? rabbit etc?
There has been no response from the company to date.
Dave Gebhard
Albert Einstein College of Medicine PH: 718-430-2724
FACS Facility, 348 Chanin Building FAX: 718-822-6538
1300 Morris Park Avenue
Bronx, New York 10461
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web