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I was told that it is possible to freeze cells in Fetal Calf Serum,
thaw them out, and run surface markers on them. I have some recipies for
the freezing media, but I was wondering if someone else has some experience
with doing this. What are some of the potential pitfalls? Do you see any
change in the surface antigen expression? What is the concentration of cells,
and how much can you freeze? The lowest concentration of FCS to cells that
I have seen is a 50-50 mix. What do you add as buffers? Any info would
be wonderful. Thanks in advance--robert rainer
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web