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A temporary fix (until v.2.0 is available) would be to use CellQUEST
software (making the assumption you are using a BD instrument) and doing a
histogram overlay with your normal control sample. You can then save the
overlayed histograms as a single combined histogram, and analyze this file
with ModFit LT.
Or you could spike all your samples with fresh PBMCs so a reference peak
will be in each specimen (theoretically, this is the best solution, I
believe).
Keith Bahjat
Chicago, IL
-- Keith Bahjat Northwestern University Kbahjat@nwu.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web