ploidy analysis
Arnold Mixon (amixon@helix.nih.gov)
Thu, 29 Aug 1996 15:20:53 -0400
We have just acquired Verity's version 1.0 Cell Cycle Analysis software,
Modfit LT. We want to simply look at whether or not a given fresh/cultured
tumor sample is anueploid or not. The problem is unlike the old B.D.
Cellfit program, there is no way of doing a comparison analysis using a
control/normal diploid standard or reference.??? I spoke to a Verity
person who claimed they were going to correct this in their
soon-to-be-released version 2.0. In the mean time, any suggestions on how I
should make my "comparisons"? Being no expert at this, the few papers I
pulled on the subject (Dressler, 1989 and Shankey..1993 Guidelines for DNA
Flow) give conflicting instructions on whether or not it is appropriate to
MIX aliquots of normal/diploid and unknowns, and run them simultaneously (
to generate 2 distinct G0-G1 peaks). Any help or referals would be
appreciated.
Thanks,
Arnold Mixon
Arnold Mixon
National Institutes of Health
N.C.I., Surgery Branch
Flow Cytometry Lab
Tel.# 301-496-9816
E-mail: amixon@helix.nih.gov
"Just the FACS Ma'am - I only want the FACS !!"
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
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If you have any comments please direct them to
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web
http://www.cyto.purdue.edu
, EMAIL
cdrom3@flowcyt.cyto.purdue.edu
