Sticking it to PBMCs
sgaffin@NATICK-CCMAIL.ARMY.MIL
Wed, 09 Apr 97 17:22:15 EST
To All Fluorescence Imaging People:
I have an ACAS 570 and want to study peripheral blood mononuclear
cells in a thermoregulated cell superperfusion chamber. My problem is
that these cells won't stick to the glass coverslip at all unless I
use CellTak, but even when I use Cell-Tak, they float away when there
is any movement of the superperfusing fluid. As a result, I can't
change the medium surrounding the cells. I have tried the usual
adhesives like PEG, gelatin, and polylysine
Does anyone know of a "superglue" for PBMCs?
Thanks for considering this.
Steve Gaffin
US Army Research Institute for Environmental Medicine
Natick MA 01760-5007
Tel 508-233-4867
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