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Hello, all:
We are interested in counting the number of cells expressing various
cytokines in a mouse model using flow cytometry. The only published work
I've seen looking at intracellular cytokines and flow was in vitro
stimulation in the presence of a protein transport inhibitor.
Do any of you have experience detecting intracellular cytokines from in vivo
experimental sytems (such as mouse blood or spleen cells) by flow? Is it
reasonable to expect to be able to get anti-cytokine antibody molecules into
a cell without the cytokines leaking out? (I assume a fixation step would
have to preceed the permeablization step.) I'd appreciate any information
about, or directions to, a protocol which works.
Thanks in advance.
* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
* Richard K. Meister Email: Meister.1@osu.edu *
* The Ohio State University Voice: (614) 292-9716 *
* Dept. of Veterinary Biosciences FAX: (614) 292-6473 *
* Cytometry Instrumentation Lab *
* 1925 Coffey Road *
* Columbus, OH 43210 U.S.A. *
* * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * * *
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Date: Thu, 03 Apr 1997 17:16:13 -0600 (CST)
From: "Richard K. Meister" <rmeister@pop.service.ohio-state.edu>
Subject: Intracellular cytokine measurement
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web