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We have been trying to do intracellular staining for p25 protein (HIV
Ag) on HIV-infected cells. We have problems with cell loss after
PermeaFix fixation and permeabilization and with background and/or
non-specific staining (DAKO and/or Coulter p25 MoAb). Anybody out
there can help us?
Thanks in advance,
Regards to all of you.
Karolina Palucka
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web