 |
 |
 |
 |
 |
>
>I have some flow datas of healthy patients. These datas show, that
>HLA-DR negative cells have ~30 %. Other cells - HLA-DR positive are .
>
Most people would agree with Gary Woo that few if any T-cells in peripheral
blood of normal patients express HLA-DR. This is an activation antigen
which, in my experience, comes up relatively late (cells in
lectin-stimulated cultures may take 48 hours or more to express it). Ther
are always a few activated cells circulating in healthy people; perhaps 1%
of peripheral blood T-cells might express CD25, CD71, CD98, HLA-DR, and
other activation antigens. If you find 60-70% of cells from "healthy"
patients to be HLA-DR positive, either your "healthy" patients are in the
midst of a robust immune reaction or something went wrong with the analysis.
-Howard
|
|
|
|
|
|
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web