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Is this always a better idea? I did an experiment a while back in
which I used indirect ("sandwich") staining with an antibody against MHC
class I. By the time I got around to the analysis, the light scatter prop-
erties of the cells that were positive for my antibody were all out of whack,
as if I had stimulated them. It reminded me of a technique I once used for
stimulating T cells, in which you added anti-CD3-biotin and then streptavidin.
So, if cross-linking the antigen in question would stimulate your
cells, perhaps you want to use direct-labeled antibodies. Comments?
- John Ladasky
ladasky@leland.stanford.edu
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web