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> From: Wallace Lauzon <wlauzon@uottawa.ca>
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Subject: A GOOD monocyte marker?
> Date: Monday, March 10, 1997 3:11 PM
>
>
> Hi,
>
> I have a rather simple sounding question, particularly for the sorters
> out there. I am looking to positively separate CD4, CD8, and monocytes
> from Ficoll-hypaque separated blood samples. I intend to use avidin
> columns and biotinylated antibodies. Obviously CD4 and CD8 will be use
> for the isolation of these lymphocytes, but the best monocyted marker is
> less clear. I have considered CD14, CD15, and CD33. My goal is to
> obtain a pure monocyte sample, not necessarily the entire monocyte
> population. The cells will be fixed immediately after they are
> isolated and the subsequent manipulations are not affected by the
> activation state of the cells. Does anyone have experience with these
> surface markers or others that would be more appropriate for isolating a
> relatively pure monocyte population?
>
> Thanks in advance
>
> Wallace Lauzon
> Dept. of Microbiology and Immunology
> University of Ottawa
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
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