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Calman Prussin
Laboratory of Allergic Diseases
NIAID/ National Institutes of Health
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From: Michelle N Fiordalisi
Sent: Wednesday, February 26, 1997 9:39 AM
To: cyto-inbox
Subject: Re: BD fastimmune protocol
Since I've had several requests for a workable protocol for
intracellular
cytoking staining, I'd lot I'd post it for every one.
The procedure I've been using is based on the BD protocol published in
their Application Note 1.
[text deleted]
I then acquire 3000-5000 events in a CD3+ gate and analyze by
quadrant analysis (CellQuest) of dot plots. I calculate % positivity
using BD's formula: (Activ. sample - activ. isotype control) - (
Unstim.
sample - Unstim. isotype control).
-- Michelle
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Michelle N. Fiordalisi, Ph.D.
Clinical Immunology Fellow
University of North Carolina Hospitals
email: mnfiord@med.unc.edu
Phone: 919-966-4058 FAX: 919-966-0486
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web