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Ethanol also is a most effective fixative agent (!) & as such will
fix any residual protein, et. al. on to the walls of your instrument's
tubing, flow cell, etc. very efficiently as well!
"Zip".
>>>--->
----------------------------------------------------------------------
> Date sent: Mon, 24 Feb 1997 11:44:40 +0000
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> From: Arnold Pizzey <a.pizzey@ucl.ac.uk>
> Subject: Clean machines
>
>
> Greetings all.
>
>
> I would question the efficacy of using 70% ETOH alone as a
> sterilization agent specifically in flow as well as generally in the
> laboratory. Although ETOH has some bactericidal activity against common
> water-borne contaminants such as the pseudomonads and the like I would
> imagine that most airborne contaminants that float into the sheath tank
> would be of the Gram-positive persuasion and as such quite resistant to the
> effect of ETOH.
> As for swabbing down external surfaces with 70% ETOH, I would regard this
> as an excellent strategy for mobilizing bacterial aerosol.
>
> On the few occasions when I have had to carry out sterile sorting, my
> strategy is as follows:
>
> Clean sort nozzle/flow cell /sheath tank with a proprietary detergent and
> rinse in sterile H2O.
> Wash the sort collection area down with a proprietary detergent
> Bypass the in-line sheath filter, Then flush system with following:
> 1)H2O
> 2)Surface active agent e.g. 0.1% DECON 90
> 3)Sterile H20
> 4)Sterile sheath fluid
> 5)Fit new sterile in-line sheath filter.
>
> Regards .
>
> Arnold.
> _____________________________________________________
> Arnold Pizzey
> Department of Haematology
> 98 Chenies Mews
> London WC1E 6HX
> E-mail a.pizzey@ucl.ac.uk.
> Voice (0)171 209 6234;
> fax (0)171 209 6222.
> _____________________________________________________
>
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web