RE: WEHI-231 sensitivity to complement deoendent killing

Dan Smith (DJS@ALLP.COM)
Wed, 19 Feb 97 09:30:00 PST

Certain strains of WEHI cells are very sensitive to TNF (nm range) if that
is any help.
/Dan Smith
djs@allp.com
----------
{From: owner-cyto-sendout
{To: Cytometry Mailing List
{Subject: WEHI-231 sensitivity to complement deoendent killing
{Date: Tuesday, February 18, 1997 7:43PM
{
{
{Has anyone had any experience killing WEHI 231 cells with antibody and
{complement?
{We want to use this as a technique for selecting cell surface molecue loss
{variants but find that the cells are difficult to kill. Anyone who has
{had success (or failure) would have information of use to us.
{Thanks in advance.
{
{Henry H. Wortis
{Department of Pathology
{Tufts University School of Medicine
{136 Harrison Ave.
{Boston MA 02111-1800
{(617)-636-6718
{FAX (617)-636-8590
{
{


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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone: (765)-494-0757; FAX(765) 494-0517; Web http://www.cyto.purdue.edu , EMAIL cdrom3@flowcyt.cyto.purdue.edu