Re: beads

Gerhard Nebe-von-Caron (Gerhard.Nebe-von-Caron@unilever.com)
12 Feb 1997 13:04:08 Z

Depending on the purpose of it you might get a way with
surface labeling some yourself with FITC, but even that is
not only green.

Otherwise you could try:

Bangs Laboratory at
http://www.bangslabs.com/

Molecular probes at
http://www.probes.com/

prolabo about estapor beads on
http://www.prolabo.com/products/esta.htm

seradyn under
http://www.seradyn.com/particletech.html

We use 0.5u coumarin labeled beads (yellow-green) as
counting standard for the bacteria. The samples are
routinely run in 0.05% tween20 to keep the CV's low. That
also reduces carryover and does not effect the cell
viability.

Regards

Gerhard Nebe-v.Caron
Unilever Research, Colworth,
Sharnbrook, Bedfordshire
GB - MK44 1LQ
Tel: +44(0)1234-222066
FAX: +44(0)1234-222344
gerhard.nebe-von-caron@unilever.com

______________________________ Reply Separator _________________________________
Subject: beads
Author: agawin@azathoth.ceab.es at INTERNET
Date: 12/02/97 02:44

Hello to all flow-ers:

Thanks to all those who replied on cleaning the machine after passing <2 um
beads. One more question : where can we buy beads that fluoresce ONLY in
green, around 1 um size. We asked Polyscience and they say that these beads
are to be custom made. We would appreciate any info. on where to look for
these. Thanks in advance!

Nona Sheila R. Agawin
Centro de Estudios Avanzados de Blanes (CEAB)
Consejo Superior Investigaciones Cientificas (CSIC)
Cami de Sta. Barbara s/n
17300 Blanes, Girona
Spain
FAX: 34 72 337806
Phone: 34 72 336101
WWW page: http//www.ceab.es


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