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What sheath fluid are you using? Commercial sheath buffer isn't great for cell
viability, use DPBS or HBSS and sort directly into medium with 20% FCS.
Best of luck
Simon
ADARC
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web