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I was wondering whether anyone has tried determining the copy number
of a gene inserted into the nucleus by transformation. We are trying
to determine the copy number of a gene which we have
transformed into tomato plants. This gene interferes with PCNA
resulting in endopolyploidy. I would therefore like to see if I
could correlate copy number with ploidy level, and
what is the expression level of PCNA in these cells.
Any suggestions would be most welcome,
Thanks
Iona O'Brien.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web