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A while back (last year?!) we were doing a quite a bit of work with trypan
blue for use as a counterstain. I presented a poster and a talk in
Charleston where we demonstrated this technique for use with surface
antibodies, in situ PCR, and in situ hybridization. We have successfully
used it in single and dual color (FITC/PE) staining. The paper has been
submitted, but if you'd rather get the info sooner than later, I'd be more
than happy to share our protocol, and experiences with you.
Drop me an e-mail.
VLM646@nwu.edu
Viki Mosiman
RHLCC Flow Cytometry Core Research Facility
Northwestern Univ
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web