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I=B4m interested in detection/quantification of cytoplasmatic and/or
intranuclear STAT proteins by fluorescence microscopy or flow.
Has anybody experience with these proteins? E.g.:
- Is the amount of protein/cell above the detection limit?
- Are fixation and staining protocol(s) available?
All papers I found so far describe use of anti-STAT mAbs for Western
blotting analysis etc. but not for immunofluoescence stainings.
Every suggestion is welcome!
Thanks in advance
Georg
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H.G. Kreysch
Merck KGaA
Biomed Fo/IMO - A25/410 Tel.: (49)6151 - 72 7323=20
Frankfurter Str. 250 FAX: (49)6151 - 72 7398
D-64271 Darmstadt E-Mail: kreysch@merck.de
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the
Purdue University Cytometry Laboratories and distributed free of charge
as an educational service to the cytometry community.
If you have any comments please direct them to
Dr. J. Paul Robinson, Professor & Director,
PUCL, Purdue University, West Lafayette, IN 47907.
Phone: (765)-494-0757;
FAX(765) 494-0517;
Web