CMV antigen detection in PMN

Adrian O Vladutiu (vladutiu@ACSU.Buffalo.EDU)
Tue, 24 Dec 1996 12:55:05 -0500 (EST)

Hello everyone,
We would like to use flow cytometry to detect (and quantitate) the PMN
cells that have in them an immediate-early antigen for CMV. The test is
performed for many transplant recipients who may be acutely infected with
CMV. The technique used so far (fluorescent microscopy counting 50,000
separated PMN cells) is laborious and inaccurate. Flow cytometry (after
permeabilizing the cells) would be much better. Anyone with experience or
suggestions? The test is valuable for many patients.
Thank you and Happy Holidays.