For a method for flow detection of cytokine mRNA, try Morvan, PY et al (1994)
"In situ hybridisation and cytofluometric analysis of cytokine mRNA during in vitro
activation of human T cells," European Cytokine Network 5 (5): 469- 480.
I'm interested in this too, but probably won't have a chance to try it for a couple of
months - please let me know how you get on! If it worked well it would be a lot
cheaper (and more versatile) than using MoAb's.
Lachy McLean
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Lachy McLean
Senior Lecturer in Clinical Immunology
Department of Molecular Medicine, University of Auckland
Private Bag 92019, Auckland 1, New Zealand
Tel: 64-9-373-7599 ext 6170 Fax 64-9-373-7492
EMail: l.mclean@auckland.ac.nz