Calman Prussin
>----------
>From: Janek Brozek
>Sent: Tuesday, December 3, 1996 13:04
>To: Cytometry Mailing List
>Subject: Re: PE-Cy5 or PER-CP
>
>
>As far as I know there is no commercial source of anti-human or anti-mouse
>immunoglobulins directly conjugated to PE-Cy5 or PerCP. There are ways to
>bypass it, though.
>1. Get the biotinylated antibody and the streptavidin conjugated PerCP from
>Becton Dickinson, or streptavidin-PE-Cy5 from, say DAKO. The best source of
>biotinylated goat anti -human/-mouse MoAbs is Jackson ImmunoResearch.
>
>2.Get those anti-immunoglobulin MoAbs conjugated to FITC or PE and the other
>ones you want to use (in a 3 color setup) conjugated to PerCP or PE-Cy5.
>This idea does not work if you need ALL of your MoAbs (FL1,2,3) directed
>against immunoglobulins, of course.
>
>Hope this helps a bit,
>
>Janek
>
>--------------------------
>mmbrozek@cyf-kr.edu.pl
>http://www.cyf-kr.edu.pl/~mmbrozek
>
>