Re: FACScan FLx drift
Joseph Webster (J.Webster@centenary.usyd.edu.AU)
Mon, 02 Dec 1996 15:22:46 +1000
At 05:34 PM 28/11/96 +0000, Andy Riddell wrote:
>Hi Eric,
>I had a similar problem on my FACSCalibur to your FLx drift. It turned out
>to be one of the hydrophobic air pressure filters behind the fluidics control
>panel got wet and an 'air lock' of sorts formed. I just replaced the filter and
>it worked fine. As to how the filter got wet, I haven't yet tracked down the
>cause.
>
A possible cause is an overfilled sample tube!
If a full tube is put on the sample uptake position, it is possible to
force fluid up the air line, and the first barrier the fluid meets is
that filter.
Cheers, Joseph.
Joseph Webster (O.I.C. Flow Cytometry)
Centenary Institute of cancer Medicine & Cell Biology
Locked Bag No.6, Newtown, NSW 2042, AUSTRALIA.
Ph: 61-2-9565-6110 Fax: 61-2-9565-6101