I sent last week this message about DNase:
"I'm trying to degrade the DNA in photosynthetic prokaryotic cells. I
want to keep the stucture of the cell, RNA and chlorophyll. This prevent the
use of detergent. In all the tests I have performed, 50% or more of the
photosynthetic population look non-sensitive to the action of DNase. Have I
a chance to obtain 100% of DNA-degradation in my photosynthetic cells ?"
I forgot to mention that my cells are fixed with para-formaldehyde,=
=20
then freezed in liquide nitrogen and stored at -80=B0C until analyze. =
=20
Thanks for help. Best regards.
Dominique Marie
e-mail: marie@sb-roscoff.fr