Austin Lin enquired :
>>I need some advices regarding the use of ModFit S/W.
>>For example, one set of data can
>>have 10% G0/G1, 80% S and 10% G2/M. The s/w scans three peaks and take the
>>second S peak as G2/M peak. Another example will be a set of data with 20%
>>G0/G1 and 80% S and invisible G2/M. The s/w will scan and see only one
>>peak.
>>
>>Would anyone suggest a good
>>Mac-s/w for us? (We have a FACScan and Macintosh Quadra 650) Thank you in
>>advance.
>>
>
>I have recently processed several hundred histograms with 'cylchred'.
>Some of these have been BrdU +ve/-ve fractions following pulse
>labelling. Many of these fit the description above and others have
>been more extreme. The program will attempt to find G0/1 G2/M
>positions, if you are not happy with these positions values can be
>called back from the previous histogram or inserted manually.
>Calculation of phases is then based on Watson et al Cytometry 8 1-8
>which handles synchronised populations as well as more conventional
>distributions.
>Version 1.0.5 uses wildcards for easy selection of groups of files
>and produces a textfile of results for transferring into
>spreadsheets. It is PC based but I have seen it operating on a Mac
>with SoftWindows.
>
>Copies from
>http://www.cf.ac.uk/uwcm/hg/hoy/
>That takes you to my home page which starts with an
>illustration from Cylchred
>Follow the links to 'Software from Cardiff'
>
>If you prefer ftp
>OPEN ftp.cf.ac.uk
>CD /pub/UWCM/hg
>GET cylchred.exe (as binary).
>
>Terry.
>
>Dr. Terry Hoy
>Principal Research Officer
>Department of Haematology Telephone 44 1222 743458
>University of Wales College of Medicine FAX 44 1222 744655
>Heath Park E-mail hoy@cardiff.ac.uk
>CARDIFF CF4 4XN http://www.cf.ac.uk/uwcm/hg/hoy/index.html
>U.K.
Gerald E. Marti
Flow and Image Cytometry Section
Laboratory of Medical and Molecular Genetics
Division of Cell and Gene Therapies
CBER FDA NIH Bdg 29B Rm 502
8800 Rockville Pike
Bethesda,MD 20892
301-827-0453 voice
301-827-0449 fax