>
> I would like to post the following message on the flow cytometry
> listserv:
>
> Does anyone routinely use cytoplasmic CD3 or cytoplasmic CD22 for acute
> leukemias? If so, what has been your experience? Are these markers
> helpful? We are considering adding these to our panel.
>
> Tim Singleton, MD
> singleto@umich.edu
>
Hi, Tim, regarding your question on intracytoplasmic CD3 and CD22, I am
routinely staining for these antigens in combination with
myeloperoxidase. We are using Caltag's FIX & PERM reagents for fixation
and permeabilization and have good experience.
Elisabeth Paietta