Since its getting cold here in Chicago, and to add a bit more fuel to
that fire... We have run those same pesky beads on our Epics (I call it a
minus V, since it's a 541) and on an XL numerous times, and in each
instance we get separation of the six different bead populations that is
at least comperable to that shown in Fig. 4 (A,B, or D) of that now
famous CCC article (Chance, et al) in both the FITC and PE channels. From
my experience, most flow cytometers that are properly maintained
(including filters that actually filter, clean optics, stable fluidics)
and operated give good separation of these types of beads.
This thread brings up the uncomfortable issue of - how are we EVER going
to have a serious impact on clinical applications if we can't even
overcome the basics?
Vince Shankey
Loyola University Med Cntr.