RE: PI staining and washing
TIM KUTE (tkute@bgsm.edu)
Wed, 9 Jul 1997 17:12:06 -0400 (EDT)
To all:
Just as a comment about PI staining. It is not necessary to wash
out the excess stain of PI. It is known that PI is not very fluorescent
unless it is bound to DNA. Therefore, the unbound PI will not be measured
by flow cytometry.
Dr. Tim Kute email: tkute@bgsm.edu
Associate Professor in Pathology Tel#: 910-716-2950
Wake Forest Univ-Bowman Gray School of Medicine Fax#:910-716-7595
Medical Center Blvd
Winston-Salem, NC 27157-1072