We would like to stain human PBL for simultaneous dual color surface
markers, and IFN gamma intracellular expression, using a coulter
epics profile 2. We would like to quantitate IFN -G production by
memory T cells and CD4/8 subsets in this way. Most of the commercial
mca to surface markers will be FITC/PE combinations, does anyone know
of any available IFN-gamma MCA conjugated to a third fluorochrome
which could be used in these experiments? I understand that for I.C.
work PE should be used, not FITC, but this won't work as PE-conjugated
MCA will be used for surface marker as indicated.
Any advice?
Thanks,
Stan.
Stan Ress
Clinical Immunology laboratory
Dept of Medicine
University of Cape Town
Cape Town, South Africa
e-mail: sress@uctgsh1.uct.ac.za
Phone: Intern + 2721-4066201
FAX : Intern + 2721-4486815