RE: 1.Instrument settings 2.saponin

Eric Martz (emartz@microbio.umass.edu)
Wed, 05 Jul 1995 12:07:19 -0500 (EST)

In message Tue, 04 Jul 1995 19:14:57 +0200,
Mikael Vestberg <Mikael.Vestberg@inflam.lu.se> writes:

> Hello everyone,
> Does anyone know how to get instrument settings from a datafile
> collected with Cellquest on the Macintosh? It was possible with the HP
> system and both should be FCS2 standard, shouldn't they?

Here is an excerpt from the on-line help of my free program MFI:

[BEGIN QUOTE]
CYTOMETER/SOFTWARE FILES TESTED
[...]
FACStation 1.0 'CellQuest' MacIntosh System Software 7.1.0 (FACScan) ***
[...]
*** The FACStation 1.0/MacIntosh files tested do not include instrument
gains, PMV's, threshold, or compensation. Becton-Dickinson promises to
rectify this serious deficiency in a future upgrade. This deficiency
precludes MFI from detecting inadvertant changes in instrument settings.
[END QUOTE]

The above statement is based on my September, 1994 correspondence with
Michael Cosgrove, B-D's project manager for CELLQuest. I have CC'd this
message to Mr. Cosgrove -- perhaps he can let us know when the upgrade can
be expected?

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