DNA + 3-color surface staining

Steve Neben (SNeben@genetics.com)
Thu, 09 Jan 1997 14:31:23 -0500

Dear Flow-ers:

I'm looking for a good method for staining bone marrow cells for
simultaneous cell cycle analysis and 3-color surface
immunofluorescence. Antibodies are FITC-, PE-, and biotin-conjugated.
I'm using a FACStarPlus with Argon, He-Ne, and UV lasers. Can anyone
suggest a good DNA stain and avidin conjugate, and staining method
that will work for this configuration ?

Steve Neben
Genetics Institute
Cambridge, MA