I'm looking for a realiable method of staining bone marrow cells for
simultaneous cell cycle analysis and 3-color surface
immunofluorescence. Antibodies are FITC-, PE-, and biotin-conjugated.
I'm using a FACStarPlus with Argon, He-Ne, and UV lasers. Can anyone
suggest a good DNA stain and avidin conjugate that will work for this
configuration ?
Steve Neben
Genetics Institute
Cambridge, MA