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Flow and Image Cytometry
Servicio General de Citometria
Laboratorio
Hospital Universitario de Salamanca
37007 Salamanca, SPAIN
Laboratory Profile
Alberto Orfao, MD, PhD
1.Facilities available: 100 m 2 facility containing instrumentation, computer facilities and lab facilities.
2. Instrumentation:
Flow Cytometry: Becton Dickinson FACStar with an ILT argon ion air-cooled laser; FACScan with Consort 32 software; FACSPrep with Consort 30 software.
Image Cytometry: Becton Dickinson Discovery image cytometry system with fluorescence and absorption microscopy.
Other major items: Shandon Cadenza automated preparation systems for slide smears and cytocentrifuge preparations; Other items available in cytometry lab (centrifuges, fluorescence microscopes, IBM PC computers, etc.)
3. Specialization areas: Leukemia/lymphoma immunophenotyping; IUV immunophenotyping; DNA analysis; FISH methodology; Platelets; Proliferation-associated assays; Reticulocytes; Mitochondrial studies; Analysis of pancreata zymogen granules.
4. Training Opportunities: All dealing with the previous items, including sample preparation, acquisition/ analysis of data and data interpretation.
5. Special courses offered: Detection of minimal residual disease in acute leukemias; Recent applications of flow cytometry; FISH technology; Applied FISH; Cell sorting techniques, Quality assurance in clinical flow cytometry; Principles and application of flow cytometry (basic course). Most of the courses are included within the EC Comett II Program.
6. Graduate Program: Included in the Spanish PhD program.
7. Local Accomodations: University facilities from 25-40 $ per night. There are also inexpensive hotels
(less than 130$ per week), breakfast usually included. In all cases, the lab facilities can be reached by 5 min walking.
8. Transportation: Closest major airport is Madrid-Barajas (MAD), There is a bus service connecting
Madrid and Salamanca every hour (25$ each way).
9. Cost of Internships: No costs. Additional support can be obtained by members of TATIANA (Trans
European Association for Training in Image Analysis) for accomodation and travel expenses,
10. Communication:
Alberto Orfao, MD, PhD
Director
Servicio General de Citometria
Laboratorio
Hospital Universitario de Salamanca
37007 Salamanca, SPAIN
Phone: (34) 23 291 375
FAX: (34) 23 264 743
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765) 494-0757; FAX (765) 494-0517; Web http://www.cyto.purdue.edu, EMAIL cdrom3@flowcyt.cyto.purdue.edu