Flow Cytometry Quality Control

 

Flow cytometry quality control requires the verification of all parameters of testing, including the instrument and other equipment, antibody staining qualities, and the lysing procedure. Instrument function checks must be customized according to the cytometer in use in the laboratory and the manufacturer's recommended function checks. Because the instruments currently in the field vary in the number and type of parameters needing monitoring, it is impossible to provide pre-printed forms that will be suitable for every instrument. The forms included in this manual may be used as guides. The important aspects to note are that all parameters being checked must have stated tolerance limits and all work must be reviewed periodically by the laboratory director.

 

Instrument Parameters

 

Cytometry Function Checks

Other Laboratory Equipment Function Checks

 

Corrective Action

 

Equipment Service Log

 

Pipette Calibration

 

Method Validation

 

Reagent Quality Control

 

Remedial Action for Test Results Reported in Error


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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765) 494-0757; FAX (765) 494-0517; Web http://www.cyto.purdue.edu, EMAIL cdrom3@flowcyt.cyto.purdue.edu