Flow Cytometry Quality Control
Flow cytometry quality control requires the verification of all parameters of testing, including the instrument and other equipment, antibody staining qualities, and the lysing procedure. Instrument function checks must be customized according to the cytometer in use in the laboratory and the manufacturer's recommended function checks. Because the instruments currently in the field vary in the number and type of parameters needing monitoring, it is impossible to provide pre-printed forms that will be suitable for every instrument. The forms included in this manual may be used as guides. The important aspects to note are that all parameters being checked must have stated tolerance limits and all work must be reviewed periodically by the laboratory director.
Instrument Parameters
Cytometry Function Checks
Other Laboratory Equipment Function Checks
Corrective Action
Equipment Service Log
Pipette Calibration
Method Validation
Reagent Quality Control
Remedial Action for Test Results Reported in Error
Back to standards & regulations
CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765) 494-0757; FAX (765) 494-0517; Web http://www.cyto.purdue.edu, EMAIL cdrom3@flowcyt.cyto.purdue.edu