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The effect of DNA damaging agents on the cell cycle can be monitored by flow cytometry. Here, a fibroblast cells line was exposed to an alkylating agent that can be seen to cause cells to arrest in S phase . With time and exposure to lower growth temperature, DNA damage is repaired and cells revert to normal asychronous growth.
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CD-ROM Vol 3 was produced by Monica M. Shively and other staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(765) 494-0757; FAX (765) 494-0517; Web http://www.cyto.purdue.edu, EMAIL cdrom3@flowcyt.cyto.purdue.edu